Hitherto Unknown [Fe-Fe]-Hydrogenase Gene Diversity in Anaerobes and Anoxic Enrichments from a Moderately Acidic Fen

Schmidt, Oliver; Drake, Harold L.; Horn, Marcus A.

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Schmidt, Oliver; Drake, Harold L.; Horn, Marcus A.: Hitherto Unknown [Fe-Fe]-Hydrogenase Gene Diversity in Anaerobes and Anoxic Enrichments from a Moderately Acidic Fen. In: Applied and Environmental Microbiology 76 (2010), Nr. 6, S. 2027-2031. DOI: https://doi.org/10.1128/AEM.02895-09

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Abstract:
Newly designed primers for [Fe-Fe]-hydrogenases indicated that (i) fermenters, acetogens, and undefined species in a fen harbor hitherto unknown hydrogenases and (ii) Clostridium- and Thermosinus-related primary fermenters, as well as secondary fermenters related to sulfate or iron reducers might be responsible for hydrogen production in the fen. Comparative analysis of [Fe-Fe]-hydrogenase and 16S rRNA gene-based phylogenies indicated the presence of homologous multiple hydrogenases per organism and inconsistencies between 16S rRNA gene- and [Fe-Fe]-hydrogenase-based phylogenies, necessitating appropriate qualification of [Fe-Fe]-hydrogenase gene data for diversity analyses. Copyright © 2010, American Society for Microbiology. All Rights Reserved.
License of this version:	Es gilt deutsches Urheberrecht. Das Dokument darf zum eigenen Gebrauch kostenfrei genutzt, aber nicht im Internet bereitgestellt oder an Außenstehende weitergegeben werden.
Publication type:	Article
Publishing status:	publishedVersion
Publication date:	2010
Keywords english:	Bacterial Proteins, DNA, Bacterial, DNA, Ribosomal, Genetic Variation, Hydrogenase
DDC:	570 \| Biowissenschaften, Biologie