dc.identifier.uri |
http://dx.doi.org/10.15488/40 |
|
dc.identifier.uri |
http://www.repo.uni-hannover.de/handle/123456789/58 |
|
dc.contributor.author |
Gryshkov, Oleksandr
|
|
dc.contributor.author |
Pogozhykh, Denys
|
|
dc.contributor.author |
Hofmann, Nicola
|
|
dc.contributor.author |
Pogozhykh, Olena
|
|
dc.contributor.author |
Müller, Thomas
|
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dc.contributor.author |
Glasmacher, Birgit
|
|
dc.date.accessioned |
2015-08-19T11:59:00Z |
|
dc.date.available |
2015-08-19T11:59:00Z |
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dc.date.issued |
2014-09-26 |
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dc.identifier.citation |
Gryshkov, Oleksandr; Pogozhykh, Denys; Hofmann, Nicola; Pogozhykh, Olena; Mueller, Thomas; Glasmacher, Birgit: Encapsulating Non-Human Primate Multipotent Stromal Cells in Alginate via High Voltage for Cell-Based Therapies and Cryopreservation. In: PLoS ONE 9 (2014), Nr. 9. DOI: http://dx.doi.org/10.1371/journal.pone.0107911 |
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dc.description.abstract |
Alginate cell-based therapy requires further development focused on clinical application. To assess engraftment, risk of mutations and therapeutic benefit studies should be performed in an appropriate non-human primate model, such as the common marmoset (Callithrix jacchus). In this work we encapsulated amnion derived multipotent stromal cells (MSCs) from Callithrix jacchus in defined size alginate beads using a high voltage technique. Our results indicate that i) alginate-cell mixing procedure and cell concentration do not affect the diameter of alginate beads, ii) encapsulation of high cell numbers (up to 10×106 cells/ml) can be performed in alginate beads utilizing high voltage and iii) high voltage (15–30 kV) does not alter the viability, proliferation and differentiation capacity of MSCs post-encapsulation compared with alginate encapsulated cells produced by the traditional air-flow method. The consistent results were obtained over the period of 7 days of encapsulated MSCs culture and after cryopreservation utilizing a slow cooling procedure (1 K/min). The results of this work show that high voltage encapsulation can further be maximized to develop cell-based therapies with alginate beads in a non-human primate model towards human application. |
eng |
dc.description.sponsorship |
DFG/EXC/62/1 |
|
dc.language.iso |
eng |
eng |
dc.publisher |
San Francisco : Public Library of Science |
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dc.relation.requires |
http://dx.doi.org/10.1371/journal.pone.0107911.g001 |
|
dc.relation.requires |
http://dx.doi.org/10.1371/journal.pone.0107911.g002 |
|
dc.relation.requires |
http://dx.doi.org/10.1371/journal.pone.0107911.g003 |
|
dc.relation.requires |
http://dx.doi.org/10.1371/journal.pone.0107911.g004 |
|
dc.relation.requires |
http://dx.doi.org/10.1371/journal.pone.0107911.g005 |
|
dc.relation.requires |
http://dx.doi.org/10.1371/journal.pone.0107911.g006 |
|
dc.relation.requires |
http://dx.doi.org/10.1371/journal.pone.0107911.g007 |
|
dc.relation.requires |
http://dx.doi.org/10.1371/journal.pone.0107911.s001 |
|
dc.relation.requires |
http://dx.doi.org/10.1371/journal.pone.0107911.s002 |
|
dc.rights |
CC BY 4.0 Unported |
|
dc.rights.uri |
http://creativecommons.org/licenses/by/4.0/ |
|
dc.subject |
Cross-linking |
eng |
dc.subject |
Cryopreservation |
eng |
dc.subject |
Marmoset |
eng |
dc.subject |
Air flow |
eng |
dc.subject |
Cell staining |
eng |
dc.subject |
Amnion |
eng |
dc.subject |
Flow rate |
eng |
dc.subject |
Vernetzung |
ger |
dc.subject |
Kryokonservierung |
ger |
dc.subject |
Callithrix jacchus |
ger |
dc.subject |
Weißbüschelaffe |
ger |
dc.subject |
Marmosetten |
ger |
dc.subject |
Luftstrom |
ger |
dc.subject |
Zellfärbung |
ger |
dc.subject |
Amnion |
ger |
dc.subject |
Flussrate |
ger |
dc.subject |
Durchsatz |
ger |
dc.subject |
Cell differentiation |
eng |
dc.subject.classification |
Vernetzung <Chemie> |
ger |
dc.subject.classification |
Kryokonservierung |
ger |
dc.subject.classification |
Zelldifferenzierung |
ger |
dc.subject.classification |
Weißbüscheläffchen |
ger |
dc.subject.classification |
Marmoset |
ger |
dc.subject.classification |
Luftstrom |
ger |
dc.subject.classification |
Amnion |
ger |
dc.subject.classification |
Durchfluss |
ger |
dc.subject.ddc |
500 | Naturwissenschaften
|
ger |
dc.subject.ddc |
600 | Technik
|
ger |
dc.title |
Encapsulating Non-Human Primate Multipotent Stromal Cells in Alginate via High Voltage for Cell-Based Therapies and Cryopreservation |
eng |
dc.type |
Article |
|
dc.type |
Text |
|
dc.relation.issn |
1932-6203 |
|
dc.relation.doi |
http://dx.doi.org/10.1371/journal.pone.0107911 |
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dc.description.version |
publishedVersion |
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tib.accessRights |
frei zug�nglich |
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