dc.identifier.uri |
http://dx.doi.org/10.15488/3904 |
|
dc.identifier.uri |
https://www.repo.uni-hannover.de/handle/123456789/3938 |
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dc.contributor.author |
Becker, Annegret
|
|
dc.contributor.author |
Leskau, Miriam
|
|
dc.contributor.author |
Schlingmann-Molina, Barbara L.
|
|
dc.contributor.author |
Hohmeier, Susanne C.
|
|
dc.contributor.author |
Alnajjar, Suhayla
|
|
dc.contributor.author |
Escobar, Hugo Murua
|
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dc.contributor.author |
Ngezahayo, Anaclet
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dc.date.accessioned |
2018-11-01T09:00:44Z |
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dc.date.available |
2018-11-01T09:00:44Z |
|
dc.date.issued |
2018 |
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dc.identifier.citation |
Becker, A.; Leskau, M.; Schlingmann-Molina, B.L.; Hohmeier, S.C.; Alnajjar, S.; Escobar, H.M.; Ngezahayo, A.: Functionalization of gold-nanoparticles by the Clostridium perfringens enterotoxin C-terminus for tumor cell ablation using the gold nanoparticle-mediated laser perforation technique. In: Scientific Reports 8 (2018), Nr. 1, 14963. DOI: https://doi.org/10.1038/s41598-018-33392-0 |
|
dc.description.abstract |
A recombinant produced C-terminus of the C. perfringens enterotoxin (C-CPE) was conjugated to gold nanoparticles (AuNPs) to produce a C-CPE-AuNP complex (C-CPE-AuNP). By binding to claudins, the C- CPE should allow to target the AuNPs onto the claudin expressing tumor cells for a subsequent cell killing by application of the gold nanoparticle-mediated laser perforation (GNOME-LP) technique. Using qPCR and immunocytochemistry, we identified the human Caco-2, MCF-7 and OE-33 as well as the canine TiHoDMglCarc1305 as tumor cells expressing claudin-3, -4 and -7. Transepithelial electrical resistance (TEER) measurements of Caco-2 cell monolayer showed that the recombinant C-CPE bound to the claudins. GNOME-LP at a laser fluence of 60 mJ/cm2 and a scanning speed of 0.5 cm/s specifically eliminated more than 75% of claudin expressing human and canine cells treated with C-CPE-AuNP. The same laser fluence did not affect the cells when non-functionalized AuNPs were used. Furthermore, most of the claudin non-expressing cells treated with C-CPE-AuNP were not killed by GNOME-LP. Additionally, application of C-CPE-AuNP to spheroids formed by MCF-7 and OE-33 cells grown in Matrigel reduced spheroid area. The results demonstrate that specific ablation of claudin expressing tumor cells is efficiently increased by activated C-CPE functionalized AuNPs using optical methods. |
eng |
dc.language.iso |
eng |
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dc.publisher |
London : Nature Publishing Group |
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dc.relation.ispartofseries |
Scientific Reports 8 (2018), Nr. 1 |
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dc.rights |
CC BY 4.0 Unported |
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dc.rights.uri |
https://creativecommons.org/licenses/by/4.0/ |
|
dc.subject |
C-CPE |
eng |
dc.subject |
AuNPs |
eng |
dc.subject |
GNOME-LP |
eng |
dc.subject |
Clostridium perfringens |
eng |
dc.subject.ddc |
500 | Naturwissenschaften
|
ger |
dc.subject.ddc |
600 | Technik
|
ger |
dc.title |
Functionalization of gold-nanoparticles by the Clostridium perfringens enterotoxin C-terminus for tumor cell ablation using the gold nanoparticle-mediated laser perforation technique |
eng |
dc.type |
Article |
|
dc.type |
Text |
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dc.relation.issn |
2045-2322 |
|
dc.relation.doi |
https://doi.org/10.1038/s41598-018-33392-0 |
|
dc.bibliographicCitation.issue |
1 |
|
dc.bibliographicCitation.volume |
8 |
|
dc.bibliographicCitation.firstPage |
14963 |
|
dc.description.version |
publishedVersion |
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tib.accessRights |
frei zug�nglich |
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