dc.identifier.uri |
http://dx.doi.org/10.15488/17207 |
|
dc.identifier.uri |
https://www.repo.uni-hannover.de/handle/123456789/17335 |
|
dc.contributor.author |
do Prado, Paula F.V.
|
|
dc.contributor.author |
Ahrens, Frederik M.
|
|
dc.contributor.author |
Liebers, Monique
|
|
dc.contributor.author |
Ditz, Noah
|
|
dc.contributor.author |
Braun, Hans-Peter
|
|
dc.contributor.author |
Pfannschmidt, Thomas
|
|
dc.contributor.author |
Hillen, Hauke S.
|
|
dc.date.accessioned |
2024-04-25T07:28:48Z |
|
dc.date.available |
2024-04-25T07:28:48Z |
|
dc.date.issued |
2024 |
|
dc.identifier.citation |
do Prado, P.F.V.; Ahrens, F.M.; Liebers, M.; Ditz, N.; Braun, H.-P. et al.: Structure of the multi-subunit chloroplast RNA polymerase. In: Molecular Cell 84 (2024), Nr. 5, S. 910-925.e5. DOI: https://doi.org/10.1016/j.molcel.2024.02.003 |
|
dc.description.abstract |
Chloroplasts contain a dedicated genome that encodes subunits of the photosynthesis machinery. Transcription of photosynthesis genes is predominantly carried out by a plastid-encoded RNA polymerase (PEP), a nearly 1 MDa complex composed of core subunits with homology to eubacterial RNA polymerases (RNAPs) and at least 12 additional chloroplast-specific PEP-associated proteins (PAPs). However, the architecture of this complex and the functions of the PAPs remain unknown. Here, we report the cryo-EM structure of a 19-subunit PEP complex from Sinapis alba (white mustard). The structure reveals that the PEP core resembles prokaryotic and nuclear RNAPs but contains chloroplast-specific features that mediate interactions with the PAPs. The PAPs are unrelated to known transcription factors and arrange around the core in a unique fashion. Their structures suggest potential functions during transcription in the chemical environment of chloroplasts. These results reveal structural insights into chloroplast transcription and provide a framework for understanding photosynthesis gene expression. |
eng |
dc.language.iso |
eng |
|
dc.publisher |
New York, NY : Elsevier |
|
dc.relation.ispartofseries |
Molecular Cell 84 (2024), Nr. 5 |
|
dc.rights |
CC BY-NC-ND 4.0 Unported |
|
dc.rights.uri |
https://creativecommons.org/licenses/by-nc-nd/4.0/ |
|
dc.subject |
chloroplast |
eng |
dc.subject |
cryo-EM |
eng |
dc.subject |
gene expression |
eng |
dc.subject |
organelle |
eng |
dc.subject |
photosynthesis |
eng |
dc.subject |
plastid |
eng |
dc.subject |
RNA |
eng |
dc.subject |
RNA polymerase |
eng |
dc.subject |
structural biology |
eng |
dc.subject |
transcription |
eng |
dc.subject.ddc |
570 | Biowissenschaften, Biologie
|
|
dc.title |
Structure of the multi-subunit chloroplast RNA polymerase |
eng |
dc.type |
Article |
|
dc.type |
Text |
|
dc.relation.essn |
1097-4164 |
|
dc.relation.issn |
1097-2765 |
|
dc.relation.doi |
https://doi.org/10.1016/j.molcel.2024.02.003 |
|
dc.bibliographicCitation.issue |
5 |
|
dc.bibliographicCitation.volume |
84 |
|
dc.bibliographicCitation.firstPage |
910 |
|
dc.bibliographicCitation.lastPage |
925.e5 |
|
dc.description.version |
publishedVersion |
eng |
tib.accessRights |
frei zug�nglich |
|