dc.identifier.uri |
http://dx.doi.org/10.15488/15716 |
|
dc.identifier.uri |
https://www.repo.uni-hannover.de/handle/123456789/15840 |
|
dc.contributor.author |
Sundermann, Julius
|
|
dc.contributor.author |
Sydow, Steffen
|
|
dc.contributor.author |
Burmeister, Laura
|
|
dc.contributor.author |
Hoffmann, Andrea
|
|
dc.contributor.author |
Menzel, Henning
|
|
dc.contributor.author |
Bunjes, Heike
|
|
dc.date.accessioned |
2023-12-12T08:31:44Z |
|
dc.date.available |
2023-12-12T08:31:44Z |
|
dc.date.issued |
2021 |
|
dc.identifier.citation |
Sundermann, J.; Sydow, S.; Burmeister, L.; Hoffmann, A.; Menzel, H. et al.: ELISA- and Activity Assay-Based Quantification of BMP-2 Released In Vitro Can Be Biased by Solubility in “Physiological” Buffers and an Interfering Effect of Chitosan. In: Pharmaceutics 13 (2021), Nr. 4, 582. DOI: https://doi.org/10.3390/pharmaceutics13040582 |
|
dc.description.abstract |
Chitosan nanogel-coated polycaprolactone (PCL) fiber mat-based implant prototypes with tailored release of bone morphogenic protein 2 (BMP-2) are a promising approach to achieve implant-mediated bone regeneration. In order to ensure reliable in vitro release results, the robustness of a commercially available ELISA for E. coli-derived BMP-2 and the parallel determination of BMP-2 recovery using a quantitative biological activity assay were investigated within a common release setup, with special reference to solubility and matrix effects. Without bovine serum albumin and Tween 20 as solubilizing additives to release media buffed at physiological pH, BMP-2 recoveries after release were notably reduced. In contrast, the addition of chitosan to release samples caused an excessive recovery. A possible explanation for these effects is the reversible aggregation tendency of BMP-2, which might be influenced by an interaction with chitosan. The interfering effects highlighted in this study are of great importance for bio-assay-based BMP-2 quantification, especially in the context of pharmaceutical release experiments. |
eng |
dc.language.iso |
eng |
|
dc.publisher |
Basel : MDPI |
|
dc.relation.ispartofseries |
Pharmaceutics 13 (2021), Nr. 4 |
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dc.rights |
CC BY 4.0 Unported |
|
dc.rights.uri |
https://creativecommons.org/licenses/by/4.0 |
|
dc.subject |
BMP-2 |
eng |
dc.subject |
Bone morphogenetic protein 2 |
eng |
dc.subject |
Chitosan |
eng |
dc.subject |
Matrix effects |
eng |
dc.subject |
Release quantification |
eng |
dc.subject.ddc |
610 | Medizin, Gesundheit
|
|
dc.title |
ELISA- and Activity Assay-Based Quantification of BMP-2 Released In Vitro Can Be Biased by Solubility in “Physiological” Buffers and an Interfering Effect of Chitosan |
eng |
dc.type |
Article |
|
dc.type |
Text |
|
dc.relation.essn |
1999-4923 |
|
dc.relation.doi |
https://doi.org/10.3390/pharmaceutics13040582 |
|
dc.bibliographicCitation.issue |
4 |
|
dc.bibliographicCitation.volume |
13 |
|
dc.bibliographicCitation.firstPage |
582 |
|
dc.description.version |
publishedVersion |
|
tib.accessRights |
frei zug�nglich |
|