Groundwater is the most unexplored element of the hydrologic cycle on the Tibetan Plateau (TP) due to harsh climate conditions. This study aims at delineating and characterizing the unexplored Zhanongtang–Ganmanong aquifer, situated in the Zhagu subcatchment of the Nam Co catchment, south-central TP. Multiple hydrogeophysical and lithological in situ field and laboratory methods are applied: depth-to-water-table measurements, grain size analysis, hydraulic empirical and field methods to estimate hydraulic conductivity (K), and analysis of electrical resistivity tomography profiles. Integration of these methods revealed the existence of a Quaternary hydrostratigraphic unit that was found to be unconsolidated, laterally heterogeneous and homogeneous over depth. The results revealed consistent K ranges of three K zones, which is in accordance with local lithology. The K ranges are applicable to other locations within the Nam Co catchment with similar lithology as in the study area without further field experiments. Permafrost was found to be absent in the study area ranging from 4730 m a.s.l. to 5200 m a.s.l. altitude. These results provide insight into the hydrogeological conditions of the TP and are useful for conceptual and numerical groundwater flow modeling to predict future changes of water fluxes and water budgets caused by climatic change, especially in remote areas.