Browsing by Subject "mRNA transfection"

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Novel suspension retroviral packaging cells generated by transposition using transposase encoding mRNA advance vector yields and enable production in bioreactors

van Heuvel, Yasemin; Schatz, Stefanie; Hein, Marc; Dogra, Tanya; Kazenmaier, Daniel; Tschorn, Natalie; Genzel, Yvonne; Stitz, Jörn (Lausanne : Frontiers Media, 2023)

To date, the establishment of high-titer stable viral packaging cells (VPCs) at large scale for gene therapeutic applications is very time- and cost-intensive. Here we report the establishment of three human suspension ...
Transgene Expression and Transposition Efficiency of Two-Component Sleeping Beauty Transposon Vector Systems Utilizing Plasmid or mRNA Encoding the Transposase

Tschorn, Natalie; van Heuvel, Yasemin; Stitz, Jörn (New York, NY : Springer, 2022)

The use of two-component transposon plasmid vector systems, namely, a transposase construct and a donor vector carrying the gene of interest (GOI) can accelerate the development of recombinant cell lines. However, the ...

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